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. 2021 Jan 1;11(3):1079–1099. doi: 10.7150/thno.49354

Figure 8.

Figure 8

Sjp40 activates AMPK through regulating miR-802/Prkaa1 axis in human HEPG2 cells. (A, D, F) qRT-PCR analysis of miR-802, Prkaa1 and Prkaa2 expression in palmitate and oleic acid-stimulated HEPG2 cells upon treatment with SEA or Sjp40 (10 µg/ml) for 24 h. (B) Conservation of miR-802 target regions in the 3'UTR of Prkaa1. (C) Luciferase report assays for 293T cells transfected with pMIR-Report Luciferase vectors carrying wild type (WT) or mutated (MuT) 3'UTR of Prkaa1 in the presence of miR-802 mimic or NC-mimic. (E) Western blot analysis of palmitate and oleic acid-stimulated AMPK and ACC protein levels in HEPG2 cells in absence or presence of SEA. (G) Protein levels of Prkaa1, Prkaa2, p-AMPK, ACC and p-ACC in palmitate and oleic acid-stimulated HEPG2 cells after stimulating with Sjp40. (H) Protein levels of CD36 in palmitate and oleic acid-stimulated HEPG2 cells after stimulating with SEA. (I) Western blot analysis of palmitate and oleic acid-stimulated CD36 protein levels in HEPG2 cells in absence or presence of Sjp40. Data are expressed as the mean ± s.e.m of 3 repeated experiments. *P <0.05, **P <0.01, ***P <0.001.