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. 2020 Dec 16;29(2):236–249.e6. doi: 10.1016/j.chom.2020.12.010

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Induction of T cell responses by LV::S_FL

C57BL/6 mice (n = 3) were immunized with 1 × 10⁷ TU of LV::S_FL or a negative control LV via i.p. injection.

(A) Splenocytes collected 2 weeks after immunization were subjected to an IFN-γ ELISPOT with 16 distinct pools of 15-mer peptides spanning the entire S_CoV-2 (1–1273 aa) and overlapping each other by 10 aa residues. SFU, spot-forming units.

(B) Deconvolution of the positive peptide pools by ELISPOT applied to splenocytes pooled from three LV::S_FL-or control-LV-immunized mice.

(C) Intracellular IFN-γ versus IL-2 staining of CD4⁺ or CD8⁺ T splenocytes after stimulation with individual peptides encompassing the immuodominant epitopes.