Figure 5.

LV::S_FL vaccination reduces SARS-Co-2-mediated lung inflammation in mice

(A) Cytometric strategy to identify and quantify distinct lung innate immune cell subsets. Lung hematopoietic CD45⁺ cells were analyzed with the use of Abs specific to surface markers or a combination of surface markers, allowing characterization of innate immune cell populations, via three distinct paths and by sequential gating. Cell populations are highlighted in gray.

(B) Percentages of each cell subset versus total lung CD45⁺ cells at 3 dpi in mice sham vaccinated or vaccinated with LV::S_FL after various prime-boost regimens versus non-infected (NI) controls. All mice were pretreated with Ad5::hACE-2 4 days prior to SARS-CoV-2 inoculation.

(C) Relative log₂ fold change in mRNA expression of cytokines and chemokines in mice sham vaccinated or vaccinated with LV::S_FL after various prime-boost regimens at 3 dpi. Data were normalized to those of PBS-treated, unchallenged controls. Statistical significance was evaluated by a two-tailed unpaired t test; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001.