Figure 2.

Tracking of transferred cells using ⁸⁹Zr-oxine PET in non-human primate models. A) Distribution of transferred CD34⁺ hematopoietic stem and progenitor cells (HSPCs) in a rhesus macaque. CD34⁺ HSPCs mobilized by plerixafor and G-CSF treatment were labeled with ⁸⁹Zr-oxine and autologously transferred intravenously (44.4 kBq/10⁶ cells, 1.2 x 10⁶ cells/kg). PET/CT was performed longitudinally under continuous infusion of deferoxamine to prevent accumulation of free ⁸⁹Zr within bone. Transferred cells rapidly distribute to the bone marrow, liver, and spleen ⁶⁴. B) Distribution of transferred NK cells in a rhesus macaque. NK cells purified from peripheral blood were expanded ex vivo with IL-2, labeled with ⁸⁹Zr-oxine, and autologously transferred intravenously (15.2 kBq/10⁶ cells, 21.9 x 10⁶ cells/kg). Cell migration was longitudinally tracked by PET/CT. Deferoxamine was continuously infused during the entire imaging study. Transferred NK cells initially distribute to the lungs, followed by gradual distribution to the liver and spleen. Little distribution of NK cells to the bone marrow is observed. Adapted from ⁶⁴.