Figure 10.

Viability test of multimodal drug treatment applying the RIST or TIST protocol in neuroblastoma cell line (A) Kelly and (B) IMR-32. Control: Vehicle (DMSO)-treated cells. Cells were seeded and treated with RIST, respectively TIST combination therapy as shown in Figure 10. Cells were seeded 24 h before drug treatment with a combination of Dasatinib with Torin-2 or Rapamycin, respectively. Drug concentrations used for the combination treatments in Kelly [D+T (TIST) = 1.5 μM + 9 nM; D+R (RIST) = 5 μM + 2 μM; SN-38+TMZ = 1 nM + 225 μM] and IMR-32 [D+T (TIST) = 1 μM + 6 nM; D+R = 0.05 μM + 0.13 μM; SN-38+TMZ = 0.4nM + 120 μM]. At day 8, the cell viability was tested by applying the MTT test as described under Methods. The cell viability was normalized to control-treated cells.