Figure 11.

Immunoblot (IB) analysis of apoptotic marker expression (PARP cleavage) after multimodal drug treatment applying the RIST or TIST protocol, respectively, in neuroblastoma cell line Kelly and IMR-32. C: Vehicle (DMSO)-treated control cells. GAPDH served as loading control. Drug concentrations used for the combination treatment in Kelly [D+T (TIST) = 1.5 μM + 9 nM; D+R (RIST) = 5 μM + 2 μM; SN-38+TMZ = 1 nM + 225 μM] and IMR-32 [D+T (TIST) = 1 μM + 6 nM; D+R = 0.05 μM + 0.13 μM; SN-38+TMZ = 0.4 nM + 120 μM]. At day 8 cells were harvested and cell lysates were analyzed.