Figure 2.

Immunoblot for cell cycle regulator Cyclin D1 and p21. Kelly cells were treated with IC₅₀ concentration of Torin-2 (T) [IC₅₀ = 12 nM] or Rapamycin (R) [IC₅₀ = 30 μM]. IMR-32 cells were treated with IC₅₀ concentrations of Torin-2 [IC₅₀ = 30 nM] or Rapamycin [IC₅₀ = 40 μM]. C: DMSO-treated control cells. GAPDH was applied as loading control. Numbers below bands state protein expression normalized to GAPDH. Cells were harvested 24 h (Cyclin D1), respectively 72 h (p21) after treatment.