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. 2021 Jan 1;18(1):137–149. doi: 10.7150/ijms.48393

Figure 4.

Figure 4

Immunoblot for autophagy marker LC3B-II. (A) Kelly cells were treated with IC50 and increasing concentrations of Torin-2 [IC50 = 12nM] or Rapamycin [IC50 = 30μM]. (B) IMR-32 cells were treated with IC50 and increasing concentrations of Torin-2 (T) [IC50 = 30nM] or Rapamycin (R) [IC50 = 40μM]. C: DMSO-treated control cells. Numbers below bands state ratio between autophagy marker LC3B-II/I. GAPDH was applied as loading control.