Figure 5.
Knockdown of DLEU2 expression suppresses the expression of proliferation and metastatisis markers in CRC. After transfection with si-NC or siR- DLEU2 for 48 h, (A-B) mRNA and protein expression levels of cyclin dependent kinase 6 (CDK6) and cyclin-dependent kinase inhibitor 1A (CDKN1A) in HT29 cells were analyzed by qRT-PCR, western blotting and densitometry; (C-D) the mRNA and protein expressionof ZEB1 and ZEB2 in HT29 cells were analyzed by qRT-PCR, western blotting and densitometry. Data are shown as means ± SEM. *p < 0.05, **p < 0.01 compared with controls.