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. 2021 Jan 1;12(2):518–529. doi: 10.7150/jca.51049

Figure 4.

Figure 4

HMGB1 upregulated the expression levels of cyclin D1, E1 and PCNA and downregulated expression of MMPs and EMT. HGC-27 cells were transfected with GFP-HMGB1 or negative plasmids for 24 h (A) or HMGB1 shRNA and negative plasmids for 48 h (B) and levels of cyclin D1, E1 and PCNA were measured by Western blotting. HGC-27 cells were transfected with GFP-HMGB1 or negative plasmids for 24 h (C) or HMGB1 shRNA and negative plasmids for 48 h (D) and expression levels of N-cadherin, E-cadherin, MMP-2 and MMP-9 were determined by Western blotting. The relative levels were normalised to β-actin. Data represent mean ± SD of three independent experiments and the representative blots were shown. *p < 0.05 and **p < 0.01 vs cells transfected with negative plasmid.