Figure 8.

Inhibition of Akt, mTOR and ERK activation suppressed rhHMGB1-induced proliferation and migration in HGC-27 cells. HGC-27 cells were treated with LY29402, rapamycin and U0126 for 1 h and stimulated with rhHMGB1 for 12 h. EdU assay (A) and colony formation assay (B) were used to detect cell proliferation and wound healing (C) and transwell (D) assays were used to determine cell migration, magnification, 100×. All experiments were repeated three times and data represent mean ±SD. ^#p < 0.05, ^##p < 0.01 vs control group, *p < 0.05 and **p < 0.01 vs rhHMGB1-treated cells.