mBDNF in microdialysates of freely moving mice.
a. General workflow of a microdialysis experiment.
b. mBDNF is detectable in medial prefrontal cortex (mPFC) lysates in wild type (WT) mice, but not in BDNF conditional KO (BDNF-cKO) mice. Abs – monoclonal antibodies. I1 – Icosagen Cat.N. 100–327; I2 – Icosagen Cat.N. 100–327; I3 – Icosagen Cat.N. 100–329.
c. Neither cytosolic nor nuclear proteins appear in microdialysates of mPFC. AKT1 - RAC-alpha serine/threonine-protein kinase, H4 - histone H4.