Fig. 2.

mBDNF secretion in response to local depolarization and systemic pharmacological intervention.

Infographics show the location of the microdialysis probe.

a. Optogenetic stimulation with blue light of 10 mW (n = 4), but not with 5 mW (3) results in a sharp transient increase in extracellular mBDNF levels in the mPFC. Bar indicates the period of stimulation. * - p < 0.05, when compared between two groups in a Tukey's multiple comparison test or, when compared with baseline samples, in an unpaired Student's test.

b. KCl (60 mM)-evoked depolarization in the mPFC is accompanied by a moderate transient elevation of extracellular mBDNF levels. Bar indicates the period, during which the pulse stimulation was applied. n = 6; **** - p < 0.0001, ** - p < 0.01, when compared with baseline samples in a Tukey's multiple comparisons test.

c. S-hydroxynorketamine (10 mg/kg) evoked an increase in extracellular mBDNF content in WT (n = 4) but not in BDNF-cKO mice (n = 4). ** - p < 0.01, * - p < 0.05, when compared with baseline samples in a Tukey's multiple comparisons test.

d. S-hydroxynorketamine (10 mg/kg, n = 4) but not saline (n = 4) evoked an increase in extracellular BDNF content in C57Bl/6N mice. **** - p < 0.01, * - p < 0.05, when compared between two groups in a Tukey's multiple comparison test.