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. 2020 Jul 4;13:100239. doi: 10.1016/j.ynstr.2020.100239

Fig. 3.

Fig. 3

FKBP51-determined effect of ketamine on mBDNF secretion in the mPFC of mice.

a. Infographics describe the design of the experiment. Struggling time of WT and FKBP51–KO (KO) mice shown in a serial TST (performed 30 min, 120 min or 24 h after S-ketamine (50 mg/kg, i.p.) injection. *** - p < 0.001, ** - p < 0.01, and * - p < 0.05, when compared in a Tukey's multiple comparisons test. Number on the bars represent the group size (apparent difference is due to exclusion of two outlier values).

b. Infographics describe the design of the experiment and schematically show a probe placement. mBDNF secretion in the mPFC peaks 90 min after S-ketamine was injected in doses of 10 mg/kg, i.p., (b.1, n = 6/5) and 50 mg/kg, i.p (b.2, n = 3/3). Arrows indicate the time of drug injection. **** - p < 0.0001, ** - p < 0.01, when compared between WT and FKBP51–KO (KO) mice in a Sidak's multiple comparison test.

c. Infographics describe the design of the experiment and show the area or micro-punch dissection of the mPFC. Relative changes in the tissue content of BDNF in the prefrontal cortex after S-ketamine administration (10 mg/kg, ip). WT - wild type mice (n = 3/4), FKBP51–KO (KO) (n = 6/6) mice. . Number on the bars represent the group size.

d. FKBP51 expression specifically affect mBDNF secretion (d.1, n = 6/5), but not dopamine release (d.2, n = 4/3) evoked by KCl-stimulated depolarization in the mPFC. Bar indicates the period, during which the pulse stimulation was applied. **** - p < 0.0001, ** - p < 0.05, when compared between WT and FKBP51–KO (KO) mice in a Sidak's multiple comparison test.