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. 2020 Jul 22;13:100243. doi: 10.1016/j.ynstr.2020.100243

Fig. 4.

Fig. 4

The 3′-UTRs of synaptotagmin-1 and Bcl-2 are direct targets of miR-34a-5p in HEK 293 cells (n = 6). One of the predicted miR-34a binding sites in the 3′-UTRs of synaptotagmin-1 (A) and Bcl-2 (D) mRNA are shown. The 3′-UTR reporter assay was performed in HEK 293 cells 48 h after transfection. The reporter assay confirmed that the miR-34a mimic was capable of significantly inhibiting luciferase expression in cells expressing the wild-type 3′-UTRs of synaptotagmin-1 (B) and Bcl-2 (E). Levels of the synaptotagmin-1 (C) and Bcl-2 (F) mRNAs and proteins were reduced in HEK 293 cells treated with the miR-34a mimic. ##p < 0.01 compared with the wildtype mimic control group and **p < 0.01 compared with the wildtype miR-34a mimic group. Two-way ANOVA results of (B) Genotype F(1,20) = 24.77, p < 0.01; Treatment F(1,20) = 36.55, p < 0.01; Interaction F(1,20) = 16.64, p < 0.01; (E) Genotype F(1,20) = 49.56, p < 0.01; Treatment F(1,20) = 34.30, p < 0.01; Interaction F(1,20) = 45.49, p < 0.01.