Table 2.
Two-way ANOVAs were used to determine the effect of alcohol exposure and its interaction with early-life adversity. Data were obtained in C57BL/6J males raised under Control or LBN conditions, exposed to chronic alcohol drinking combined or not with chronic intermittent ethanol vapor inhalation (Air-water, Air-2BC, CIE-2BC), and withdrawn from alcohol.
| Test | Withdrawal timepoint (after last vapor) | Measure | Effect of alcohol | Alcohol × LBN interaction |
|---|---|---|---|---|
| Elevated plus-maze | 10–11 days | % time on open arms (Fig. 3A) | F2,40 = 3.3, p = 0.048 | F2,40 = 2.7, p = 0.080 |
| Total distance traveled (Fig. 3B) | F2,40 = 1.1, p = 0.36 | F2,40 = 1.0, p = 0.37 | ||
| Digging | 13 days | Digging duration (Fig. 3C) | F2,40 = 5.5, p = 0.008 | F2,40 = 0.1, p = 0.91 |
| Splash | 17 days | Number of grooming bouts (Fig. 3D) | F2,40 = 1.3, p = 0.28 | F2,40 = 3.7, p = 0.033 |
| Tail suspension | 19 days | Immobility duration (Fig. 3E) | F2,40 = 4.18, p = 0.023 | F2,40 = 3.1, p = 0.056 |
| Tail pressure | 32 h | Nociceptive threshold (Fig. 3F) | F2,40 = 4.3, p = 0.02 | F2,40 = 0.2, p = 0.83 |
| Novel object recognition | 6 days | Discrimination index (Fig. 3G) | F2,38 = 0.04, p = 0.96 | F2,38 = 0.6, p = 0.57 |
| Corticosterone | 7 days | Plasma levels (Fig. 3H) | F2,39 = 0.6, p = 0.55 | F2,39 = 1.4, p = 0.26 |