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Immunofluorescence for hM4Di/mCherry and cannula placements. Representative examples of neurons in the aPVT and pPVT transduced with hM4Di/mCherry (A) and fibers in the NAcSh (B). (C) Locations of the injector cannula in the NAcSh. Scale bars: A, 200 μm; B, 500 μm. Abbreviations: 3 V, third ventricle; ac, anterior commissure; aPVT, anterior PVT; Hb, habenula; LV, lateral ventricle; NAcC, core of NAc; NAcSh, shell of NAc; pPVT, posterior PVT; PT, paratenial nucleus of the thalamus.
