Fig. 8.

Number of double-labeled cFos neurons in the NAcSh expressing opioid neuropeptides following the social approach-avoidance test. (A) For dynorphin (DYN), the number of cFos+/DYN + double-labeled NAcSh neurons per mm². (B) For enkephalin (ENK), the number of cFos+/ENK + double-labeled NAcSh neurons per mm². (C) Representative images of the NAcSh showing cFos+ (green) and ENK+ (blue), and DYN+ (red) neurons in an hM4Di + Sal and an hM4Di + Clz case. Data are means ± SEM. **p < 0.01. Group size n = 4 cases for each bar. Scale bars, 25 and 50 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)