U50,488 suppressed glutamatergic transmission onto LHb neurons. A. Top: representative AMPAR-mediated mEPSC traces recorded from LHb neurons before (baseline, black) and after U50,488 (10 μM, red) application. U50,488 had a trending decrease in the mean amplitude of mEPSCs but significantly decreased cumulative probability of mEPSC amplitude. B. U50,488 treatment decreased the average mEPSC frequency and cumulative probability of inter-event intervals of mEPSCs. #p = 0.066, **p < 0.01, ***p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)