Fig. 3.

Venn diagrams of differentially expressed genes (q < 0.1). a) The intersection of DEGs in stressed vs. unstressed neurons in each pathway were few; Sema4d was the only gene to be downregulated after stress in all pathways. b-d) Pairwise comparisons between RNA from the unstressed conditions revealed few differences between the pathways although the DRN and VTA pathways differed the least from each other and shared the most DEGs relative to RMTg. e-g) RNA in the RMTg-projecting neurons from stressed animals diverged from the other pathways. While the RNA from the DRN and VTA pathways of stressed animals were similar to one another, RNA in the RMTg-projecting neurons had ∼4.5 fold more DEGs than in the comparisons from unstressed animals.