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RTqPCR validates downregulation of PI3-Kinase related genes in RMTg-projecting LHb neurons. Based on the RNAseq analysis, we expressed RiboTag in the pathway from LHb to RMTg in a new cohort of control rats (n = 6) and rats subjected to forced swim stress (n = 7). RTqPCR of the ribosome-associated RNA showed that Kdr (a) and Tek (b) were significantly reduced after stress; there was a trend for stress to reduce Ptpn13 (c). ∗p < 0.05.
