Fig. 6.

Representative images of immunostaining for Iba-1 in the NAcc, DR, PVN, and DG (A-H). Social isolation alters microgliosis in a sex- and brain region-dependent manner (I). Isolated females had significantly higher levels of microgliosis, as indicated by increased Iba-1 cell counts, in the NAcc and in the DG compared to cohoused control females. Isolated subjects had significantly higher levels of microgliosis in the DR. Males had significantly higher levels of microgliosis in the DR and in the PVN in comparison to females. Iba-1, ionized calcium binding adaptor molecule 1, NAcc, nucleus accumbens, DG, dentate gyrus, DR, dorsal raphe, PVN, paraventricular nucleus of the hypothalamus. Bars indicate mean ± SEM. Bars with different letters differ significantly from each other. ** represents a main effect of sex, p < 0.01. # represents a main effect of treatment, p < 0.05. Scale bar = 100 μm.