Fig. 3.

Sex differences in tonic eCB activity at CA1 dendritic synapses. a) Raw traces from single experiments and b) time course of averaged experiments demonstrate that FAAH inhibition (URB597, 1 μM) increases female but not male fEPSP, thus revealing tonic AEA activity at female synapses. c) Summary of the URB experiments comparing mean ± SEM baseline responses (last 5 min of the baseline period) and mean ± SEM post-URB responses (last 5 min of the experiment). *s indicate significant differences from baseline responses; #s indicate significant differences between females and males (p ≤ 0.05), mixed, two-way rmANOVA). d) Raw traces from single experiments and e) time course of averaged experiments showing increases in both female and male fEPSPs following MAGL inhibition (JZL184, 100 nM). f) Summary of the JZL experiments. g) Activation of TRPV1 with Capsaicin (5 μM) decreases female fEPSPs. n = number of slices per experiment. Each * indicates significant differences from baseline responses; #s indicate significant differences between females and males (p ≤ 0.05), mixed, two-way rmANOVA.