Fig. 6.

Raw open arm time is significantly increased by activation of the LC→mPFC projection. Because test duration varied between rats whose LC→mPFC pathway was activated as a result of falling from the EPM, several measures of open arm behavior were assessed for the time before rats fell. The duration of the first visit to the open arms, or the latency to exit the open arms after first entry, was significantly greater in rats whose LC→mPFC projection was activated (n = 4) than not activated (n = 4; A). The average duration of visits to the open arms was also significantly increased by pathway activation (B). Data were also binned in 60s increments, and rats whose LC→mPFC projection was activated spent significantly more time in the first 60s bin and the second 60s bin than rats whose pathway was not activated. No significant difference was found in the third bin when all three rats fell from the maze. No statistical comparisons could be made beyond the third bin due to only one rat remaining in this group (C). The average amount of time spent in both the open and closed arms are shown as a stacked bar graph for both groups in (D).