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. Author manuscript; available in PMC: 2020 Dec 16.
Published in final edited form as: J Mol Biol. 2016 Jan 21;428(3):590–602. doi: 10.1016/j.jmb.2016.01.011

Table 1.

Equilibrium dissociation and rate constants.

Flow Chip ka kd Kd
Activin B wtCer-Fc (2.3 ± 0.1) × 106 (2.2 ± 0.2) × 10−4 0.096 ± 0.01
mutCer-Fc (2.0 ± 0.3) × 106 (3.1 ± 0.2) × 10−5 0.016 ± 0.003
CerS-Fc (1.2 ± 0.2) × 106 (1.7 ± 0.1) × 10−5 0.014 ± 0.002
BMP-6 wtCer-Fc (5.5 ± 1.4) × 104 (1.1 ± 0.2) × 10−3 19.1 ± 2.5
mutCer-Fc (7.0 ± 1.0) × 104 (1.0 ± 0.1) × 10−3 14.7 ± 3.6
CerS-Fc (8.0 ± 0.4) × 104 (1.2 ± 0.1) × 10−3 15.2 ± 1.4
BMP-7 wtCer-Fc (1.6 ± 0.2) × 105 (2.8 ± 0.1) × 10−3 17.3 ± 0.8
mutCer-Fc (1.3 ± 0.5) × 105 (1.2 ± 0.1) × 10−3 9.8 ± 0.9
CerS-Fc (1.4 ± 0.2) × 105 (2.1 ± 0.2) × 10−3 15.6 ± 1.9

Units are presented in parentheses as follows: Ka (M−1 S−1), Kd (nM); chi2 (RU2), and kt [RU/(M s)]. Number of replicates: N = 3.