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. Author manuscript; available in PMC: 2020 Dec 16.

Published in final edited form as: J Mol Biol. 2016 Jan 21;428(3):590–602. doi: 10.1016/j.jmb.2016.01.011

Table 2.

IC₅₀ values for SPR binding inhibition and reporter assays.

SPR binding^a [IC₅₀ (nM)]

Ligand	Chip	wtCer	mutCer	CerS

Activin B	ActRIIA	26.2 ± 1.7	40.1 ± 2.5	261.0 ± 33.2
Activin B	BMPRII	14.9 ± 4.2	7.4 ± 1.1	28.4 ± 9.6
BMP-6	ActRIIA	319.0 ±53.4	350.0 ± 96.1	N.D.
BMP-6	BMPRII	156.0 ± 9.2	48.3 ± 3.5	N.D.
Reporter signaling^b [IC₅₀ (nM)]

Ligand	Cell line	wtCer-Fc	mutCer-Fc	CerS-Fc

Activin B	A-204	34.1 ± 9.4	3.7 ± 1.0	14.8 ± 1.1
BMP-6	HepG2	67.8 ± 24.0	15.8 ± 3.2	49.9 ±11.8

Five inhibitor concentrations were used.

^a

Number of replicates: N = 3.

^b

Number of replicates: N = 4.