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(A) Nucleotide exchange inhibition by covalent peptidomimetics with a fluorescent nucleotide analog (mant-GDP) as a probe. (B) Sos-mediated nucleotide exchange assays with H-Ras G12C and increasing concentrations of covalent α3βHBS 5. (C) Bar graph compares the efficacy of 5 and control peptides 6 and 7. Error bars are mean ± SD of biological duplicates.
