Table 2. . Percent recovery of dried blood spot IgG antibodies from three study subjects under simulated drying and shipping conditions at 7 days run on the (A) ZRT DBS IgG S1 and (B) ZRT DBS IgG NCP assays compared with 4 h drying at 22°C and 30% humidity, then storage in a resealable bag with desiccant at -19°C and 40% humidity†.
| (A) ZRT DBS IgG S1 | ||||
|---|---|---|---|---|
| Sample 1 | -19°C, 40% humidity (% recovery) | 5°C, 66% humidity (% recovery) | 22°C, 30% humidity (% recovery) | 29°C, 99% humidity (% recovery) |
| 4 h dry, no bag | 107.4 | 103.0 | 95.9 | 74.8 |
| 4 h dry, then in resealable bag | 108.9 | 114.4 | 101.4 | 84.8 |
| 4 h dry, then in resealable bag with desiccant | 100.0† | 103.2 | 97.2 | 92.2 |
| Wet, No Bag | 115.8 | 113.0 | 102.2 | 93.2 |
| Wet, then in resealable bag | 109.5 | 106.2 | 94.9 | 90.6 |
| Wet, then in resealable bag with desiccant | 111.5 | 107.2 | 102.1 | 89.7 |
| Sample 2 | -19°C, 40% humidity (% recovery) | 5°C, 66% humidity (% recovery) | 22°C, 30% humidity (% recovery) | 29°C, 99% humidity (% recovery) |
| 4 h dry, no bag | 106.0 | 103.7 | 95.3 | 65.2 |
| 4 h dry, then in resealable bag | 104.0 | 105.3 | 99.4 | 76.9 |
| 4 h dry, then in resealable bag with desiccant | 100.0† | 101.6 | 97.3 | 84.9 |
| Wet, no bag | 114.1 | 107.9 | 90.0 | 80.9 |
| Wet, then in resealable bag | 111.0 | 101.6 | 89.0 | 71.9 |
| Wet, then in resealable bag with desiccant | 110.4 | 114.1 | 95.0 | 86.2 |
| Sample 3 | -19°C, 40% humidity (% recovery) | 5°C, 66% humidity (% recovery) | 22°C, 30% humidity (% recovery) | 29°C, 99% humidity (% recovery) |
| 4 h dry, no bag | 103.2 | 106.1 | 105.6 | 92.3 |
| 4 h dry, then in resealable bag | 103.8 | 105.7 | 108.9 | 105.4 |
| 4 h dry, then in resealable bag with desiccant | 100.0† | 100.1 | 105.8 | 104.6 |
| Wet, no bag | 109.7 | 95.2 | 104.3 | 95.0 |
| Wet, then in resealable bag | 111.4 | 85.7 | 94.7 | 88.8 |
| Wet, then in resealable bag with desiccant | 102.7 | 93.8 | 94.2 | 92.2 |
| (B) ZRT DBS IgG NCP | ||||
| Sample 1 | -19°C, 40% humidity (% recovery) | 5°C, 66% humidity (% recovery) | 22°C, 30% humidity (% recovery) | 29°C, 99% humidity (% recovery) |
| 4 h dry, no bag | 97.8 | 97.2 | 93.7 | 84.6 |
| 4 h dry, then in resealable bag | 101.3 | 98.9 | 94.1 | 93.2 |
| 4 h dry, then in resealable bag with desiccant | 100.0† | 102.3 | 94.7 | 91.1 |
| Wet, no bag | 105.7 | 96.6 | 92.8 | 90.0 |
| Wet, then in resealable bag | 94.8 | 96.6 | 87.3 | 82.6 |
| Wet, then in resealable bag with desiccant | 102.3 | 96.7 | 94.7 | 89.5 |
| Sample 2 | -19°C, 40% humidity (% recovery) | 5°C, 66% humidity (% recovery) | 22°C, 30% humidity (% recovery) | 29°C, 99% humidity (% recovery) |
| 4 h dry, no bag | 104.3 | 97.6 | 95.9 | 74.1 |
| 4 h dry, then in resealable bag | 99.9 | 92.0 | 99.4 | 85.7 |
| 4 h dry, then in resealable bag with desiccant | 100.0† | 98.8 | 90.1 | 84.9 |
| Wet, no bag | 104.9 | 91.6 | 99.5 | 93.7 |
| Wet, then in resealable bag | 102.4 | 98.0 | 90.8 | 81.1 |
| Wet, then in resealable bag with desiccant | 104.9 | 101.4 | 97.9 | 94.0 |
| Sample 3 | -19°C, 40% humidity (% recovery) | 5°C, 66% humidity (% recovery) | 22°C, 30% humidity (% recovery) | 29°C, 99% humidity (% recovery) |
| 4 h dry, no bag | 91.5 | 100.8 | 96.9 | 97.4 |
| 4 h dry, then in resealable bag | 95.2 | 98.4 | 100.4 | 101.6 |
| 4 h dry, then in resealable bag with desiccant | 100.0† | 94.6 | 83.1 | 98.7 |
| Wet, no bag | 100.1 | 94.5 | 102.7 | 92.1 |
| Wet, then in resealable bag | 105.7 | 82.7 | 95.6 | 94.8 |
| Wet, then in resealable bag with desiccant | 102.9 | 98.2 | 98.8 | 98.9 |
†
Humidity.
DBS: Dried blood spot.