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Maximum intensity projections in a cross-sectional view of rabbit tendon over 10 z-slices. (A) SHG imaging demonstrated fascicles within the subtendons of the rabbit Achilles tendon. (B) Two-photon fluorescence microscopy revealed that WGA is present in the inter-fascicular matrix (small arrows), as well as in the region between subtendons (large arrows), indicative of an inter-subtendon matrix. (C) Overlay of (A) and (B).
