Table II.

mRNAs primers for reverse transcription-quantitative PCR.

Gene name	NCBI accession No.	Primer sequence (5' to 3')	Size (bp)
Mouse IL-1A	NP_034684	Forward: TCTGCCATTGACCATCTC	182
		Reverse: ATCTTCCCGTTGCTTGAC
Mouse TNF-α	NP_038721	Forward: GGGCTTCCAGAACTCCA	213
		Reverse: GCTACAGGCTTGTCACTCG
Mouse Nkx-2.2	NP_035049	Forward: CCGGGCGGAGAAAGGTATG	156
		Reverse: CTGTAGGCGGAAAAGGGGA
Mouse Pdx-1	NP_032840	Forward: CCCCAGTTTACAAGCTCGCT	177
		Reverse: CTCGGTTCCATTCGGGAAAGG
Mouse Gcg	NP_032126	Forward: TTACTTTGTGGCTGGATTGCTT	149
		Reverse: AGTGGCGTTTGTCTTCATTCA
Mouse Pax-6	NP_001231129	Forward: GCAGATGCAAAAGTCCAGGTG	285
		Reverse: CAGGTTGCGAAGAACTCTGTTT
Mouse Snap25	NP_035558	Forward: CAACTGGAACGCATTGAGGAA	177
		Reverse: GGCCACTACTCCATCCTGATTAT
Mouse Mafa	NP_919331	Forward: AGGAGGGTCATCCGACTG	113
		Reverse: CTTCTCGCTCCAGAATGTG
Mouse Ins2	NP_001172013	Forward: GCTTCTTCTACACACCCATGTC	147
		Reverse: AGCACTGATCTACAATGCCAC
Mouse Actin	NP_031419	Forward: GTGACGTTGACATCCGTAAAGA	245
		Reverse: GCCGGACTCATCGTACTCC

Nkx-2.2, homeobox protein Nkx-2.2; Pdx-1, pancreatic and duodenal homeobox 1; Pax-6, paired box 6; Snap25, synaptosome-associated protein 25; Mafa, transcription factor MafA; Ins2, insulin-2.