Figure 11.

Nrf2 knockdown abrogates the protective effects of eugenol on H₂O₂-induced apoptosis. 293 and NIH-3T3 cells transfected with either shNRF2 or shControl were treated with eugenol (100 µg/ml) and H₂O₂ (50 µM) for 24 h, following which western blot analysis was performed to examine the expression level of cleaved-Caspase 3 and Nrf2. Experiments were performed in triplicate. Nrf2, nuclear factor erythroid 2-related factor 2; sh, short hairpin RNA.