Figure 2.

Eugenol promotes the transcriptional activity of Nrf2 in 293 and NIH-3T3 cells. (A) 293 cells were treated with eugenol and its derivatives at the indicated concentrations. ARE luciferase reporter assay was performed 6 h later to measure the transcriptional activity of Nrf2. (B) A similar experiment was performed in NIH-3T3 cells treated with 100 µg/ml eugenol, methyleugenol or acetyleugenol. Data are presented as mean ± SD. ^*P<0.05 vs. Mock. Nrf2, nuclear factor erythroid 2-related factor 2; ARE, antioxidant responsive element.