Figure 3.

Eugenol enhanced the expression levels of Nrf2 target genes in 293 cells. (A) After 24 h treatment with 50 µg/ml eugenol and its two derivatives, mRNA expression of GCL-M and (B) GSTA1, target genes for Nrf2, was quantified by reverse transcription-quantitative PCR. (C) mRNA expression of NFE2L2, as measured by reverse transcription-quantitative PCR, after treatment with 50 µg/ml eugenol and its two derivatives. In all instances, 50 µg/ml tBHQ was applied as positive control. Data are presented as mean ± SD from three independent experiments. ^*P<0.05 vs. Mock. Nrf2, nuclear factor erythroid 2-related factor 2; GCL-M, glutamate-cysteine ligase modifier regulatory subunit; GSTA1, glutathione S-transferase A1; tBHQ, tert-butylhydroquinone.