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The content of Nrf2 protein expression was increased by eugenol. (A) 293 cells were incubated with eugenol, methyleugenol or acetyleugenol (50 or 100 µg/ml) for 24 h, followed by western blotting. (B) Nrf2 protein expression level was examined by western blotting in NIH-3T3 cells following treatment with 100 µg/ml eugenol. Experiments were performed in triplicate. Nrf2, nuclear factor erythroid 2-related factor 2.
