Figure 5.

Eugenol stabilizes Nrf2 protein in 293 and 3T3 cells. (A) 293 cells were first transfected with a plasmid expressing Nrf2 (pcDNA3.1-Nrf2) before 6 h treatment with 50 µg/ml eugenol. (B) Identical experiment was performed on NIH-3T3 cells, but with 100 µg/ml eugenol. Following CHX (20 µg/ml) treatment, the level of Nrf2 protein expression was evaluated at the indicated time points by western blotting. Experiments were performed in triplicate. Nrf2, nuclear factor erythroid 2-related factor 2; CHX, cycloheximide