Figure 7.

Nrf2 accumulated in the nucleus of in eugenol-treated cells. 293 cells with pcDNA3.1-Nrf2 or empty pcDNA3.1 plasmid were treated with eugenol (50 µg/ml), tBHQ (100 µg/ml) or Diamide (20 µg/ml) for 6 h. Western blot analysis was subsequently performed to measure Nrf2 protein levels in cytoplasmic and nuclear fractions. GAPDH and Lamin C were applied as loading controls for cytoplasmic and nuclear proteins, respectively. Experiments were performed in triplicate. Nrf2, nuclear factor erythroid 2-related factor 2; tBHQ, tert-butylhydroquinone.