Figure 8.

Eugenol protected cells against H₂O₂-induced oxidative damage. (A) 293 cells were treated with or without 50 µg/ml eugenol for 24 h. Subsequently, 10 µM CM-H2DCFDA was loaded into the cells with or without 50 µM H₂O₂. DCF assay was performed followed by flow cytometry analysis. (B) Quantified data of (A), with data presented as the mean ± SD of counts in gates R. Experiments were performed in triplicate. ^*P<0.05. DCF, 2',7'-dichlorofluorescein; CM-H2DCFDA, 2',7'-dichlorodihydrofluorescein diacetate.