Figure 3.

Effect of luteolin on MUC1 expression in gastric cancer CRL-1739 cells. Effect of (A) luteolin on MUC1 mRNA expression, MUC1 glycoproteins in (B) cell lysates and (C) culture supernatants. Cells were treated with 30 µM luteolin and H. pylori for 24 h. mRNA expression is expressed as the relative fold-change in MUC1 mRNA levels compared with the control, the expression of which was set as 1. For the ELISA of cell lysates, equivalent amounts of protein (5 µg/50 µl) was applied to each well. For culture supernatant, equivalent volumes (50 µl; 1:100) of sample was used. The results are presented as the absorbance at 405 nm after incubation with an anti-MUC1 monoclonal antibody. Data are presented as the mean ± standard deviation of three experimental repeats. ^*P<0.05, ^**P<0.01, ^***P<0.001. MUC1, mucin 1; A, absorbance; H. pylori, Helicobacter pylori.