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. 2020 Nov 24;11:559105. doi: 10.3389/fmicb.2020.559105

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Copyright © 2020 Mekonnen, Masavuli, Yu, Gummow, Whelan, Al-Delfi, Torresi, Gowans and Grubor-Bauk.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DC kinetics in DLN post vaccination. Axillary lymph nodes were harvested on days 2, 3, 5, and 7 after a single (s.c.) vaccination with 10⁶ live or necrotic NS3 DC2.4 cells CD3^–, CD11c^high, MHCII⁺ cells were identified and assessed for CD8α, CD86, and Clec9A expression. (A) Absolute numbers of CD11c^high MHC II⁺ CD8α⁺ DC. (B) Percentage of CD86 expressing CD11c^high MHC II⁺ CD8α⁺ DC. (C) Absolute numbers of Clec9A + CD11c^high CD8α⁺ DC. Plots are representative of two independent experiments (n = 5/group/time point). Data show mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01 (Mann–Whitney test).