| Antibodies |
|
|
| Anti-mouse Brilliant Violet 605, Sca-1 |
Biolegend |
Cat# 108133;RRID:AB_2562275 |
| Anti-Mouse Monoclonal eFluor 450, CD45 |
Thermo Fisher Scientific |
Cat# 48-0451-82; RRID:AB_1518806 |
| Anti-Mouse Monoclonal APC, DLK1 |
R&D Systems |
Cat# FAB8634A |
| Anti-Mouse Monoclonal PerCP-Cyanine5.5, CD26 |
Thermo Fisher Scientific |
Cat# 45-0261-82; RRID:AB_1548738 |
| Anti-Mouse Monoclonal eFluor 450, TER-119 |
Thermo Fisher Scientific |
Cat# 48-5921-82; RRID:AB_1518808 |
| Anti-Mouse Monoclonal eFluor 450, CD324 |
Thermo Fisher Scientific |
Cat# 13-3249-82; RRID:AB_1659688 |
| Anti-Mouse Monoclonal eFluor 450, TIE-2 |
Thermo Fisher Scientific |
Cat# 13-5987-82; RRID:AB_466848 |
| Anti-Mouse Monoclonal eFluor 450, CD326 |
Thermo Fisher Scientific |
Cat# 48-5791-82; RRID:AB_10717090 |
| Anti-Mouse Monoclonal eFluor 450, CD31 |
Biolegend |
Cat# 303114; RRID:AB_2114316 |
| Anti-Mouse Monoclonal eFluor 450, CD45 |
Thermo Fisher Scientific |
Cat# 48-0451-82; RRID:AB_1518806 |
| Rabbit Polyclonal, Fibronectin |
Abcam |
Cat# ab2413; RRID:AB_2262874 |
| Rabbit Polyclonal, FSP |
Abcam |
Cat# ab27957; RRID:AB_2183775 |
| Rabbit Polyclonal, Col1 |
Abcam |
Cat# ab34710; RRID:AB_731684 |
| Rabbit Polyclonal, Vimentin |
Abcam |
Cat#ab137321 |
| Rabbit Polyclonal, Col3 |
Abcam |
Cat# ab59436; RRID:AB_941099 |
| Rabbit Monoclonal, Cytokeratin 14 |
Abcam |
Cat# ab181595; RRID:AB_2811031 |
| Anti-rabbit Alexa Fluor 647 |
Thermo Fisher Scientific |
Cat# A-21443; RRID:AB_2535861 |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| Fluromont-G |
Southern Biotech |
Cat# 0100-01 |
| Ethanol |
GoldShield |
Cat# 64175 |
| Permount |
Fisher Chemicals |
Cat# SP15 |
| Triton x-100 |
Sigma |
Cat# X100 |
| DAPI-(4,6-Diamido-2-Phenylindole, dihydrochloride) |
Thermo Fisher Scientific |
Cat# D1306; RRID:AB_2629482 |
| Hematoxylin |
Sigma |
Cat# H3136 |
| Eosin |
Sigma |
Cat# HT1101128 |
| Liberase |
Sigma |
Cat# 5401119001 |
| Dulbecco Modified Eagle Medium |
Sigma |
Cat# D5796 |
| Phosphate Buffer Saline |
Sigma |
Cat# P5368 |
| Fetal Bovine Serum |
Thermo Fisher Scientific |
Cat# 10428026 |
| Tween-20 |
Sigma |
Cat# 113322465001 |
| 1x Power Block |
Roche |
Cat# Hk085-5k |
| Trypsin Antigen Retrieval Kit |
Abcam |
Cat# 970 |
| Matrigel |
Corning |
Cat# 356234 |
| Tissue Tek Optimal Cutting Temperature (OCT) |
Sakura |
Cat# 4583 |
| 1,4-Dithiothreitol |
Sigma |
Cat# 10197777001 |
| Critical Commercial Assays |
|
|
| Collagen ELISA |
Abcam |
Cat# Ab210966
|
| Chromium Next GEM Chip H Single Cell Kit |
10x Genomics |
Cat# 1000161 |
| PicroSirius Red Staining Kit |
Abcam |
Cat# Ab150681
|
| Deposited Data |
|
|
| RNA Bulk Sequencing Data |
This data has been deposited in NCBI’s Gene expression Omnibus. |
GSE159345 |
| scRNA-Sequencing Data |
This data has been deposited in NCBI’s Gene expression Omnibus. |
GSE159345 |
| Experimental Models: Cell Lines |
|
|
| Mouse B16F-10 Melanoma Cells |
ATCC |
Cat# CRL-6475 |
| Experimental Models: Organisms/Strains |
|
|
| Prrx1Cre |
The Jackson Laboratory |
JAX stock # 005584 |
| R26Mtmg |
The Jackson Laboratory |
JAX stock # 007676 |
| C57/BL/6J |
The Jackson Laboratory |
JAX stock # 000664 |
| Wnt1Cre |
The Jackson Laboratory |
JAX stock #022137 |
| R26iDTR |
The Jackson Laboratory |
JAX stock # 007900 |
| En1Cre |
The Jackson Laboratory |
JAX stock #007916 |
| Software and Algorithms |
|
|
| ImageJ |
National Institutes of Health |
RRID:SCR_003070 |
| Adobe Photoshop CC |
Adobe |
RRID:SCR_014199 |
| Adobe Illustrator CC |
Adobe |
RRID:SCR_010279 |
| Prism 5 |
Graph Pad |
RRID:SCR_002798 |
| Imaris |
Oxford Instruments |
RRID:SCR_007370 |
| MATLAB |
MATLAB |
RRID:SCR_001622 |
| Other |
|
|
| 6-0 nylon sutures |
Ethicon |
Cat# 1856G |
| Tegaderm Dressings |
3M |
Cat# 1626w |
| Telfa Dressings |
Covidien |
Cat# 2132 |
