FIGURE 4.

HCC can be regulated by interfering with DRD1 expression. (A) Western blot analysis confirmed the overexpression of DRD1 in Hep‐3B cells by stable transfection. (B) Western blot analysis confirmed the knockout of DRD1 expression in MHCC‐97H cells by stable transfection. (C) The upregulation of DRD1 expression significantly enhanced cell proliferation. (D) The downregulation of DRD1 expression weakened cell proliferation. (E) The upregulation of DRD1 expression significantly increased the colony numbers. (F) The downregulation of DRD1 expression decreased the colony numbers. (G) The upregulation of DRD1 expression significantly enhanced cell migration and invasion. (H) The downregulation of DRD1 expression weakened cell migration and invasion. The data are presented as the mean ± SD from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001.

Abbreviations: DRD1, dopamine receptor D1. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase. HCC, hepatocellular carcinoma.