FIG 5.

Growth of clone ST235 strain 60P57PA (top) and of clone ST253 strain RN3 (bottom) in batch culture. Bacteria were grown in 1.5 liters tryptone soy broth at 37°C and pH 7.0 with constant agitation (400 rpm) and supply of 0.3 liters/min compressed air. Oxygen saturation (red), redox potential (purple) (left scale), and the supply of H₃PO₄ to maintain pH 7 (right scale) were continuously monitored. Every 60 min, 1 ml was removed from the culture for the determination of OD₆₀₀ (black) and CFU (green) (right scale). Fermentations were executed twice. The two dotted lines indicate the time points when samples were taken for RNA-seq analysis of the bacterial transcriptome.