Fig. 1.

Geographic distribution of founders of wild-derived strains and their Prdm9 ZnF arrays. (A) Localities of origin of eight musculus (squares) and eight domesticus (circles) strains along the musculus/domesticus hybrid zone (Baird and Macholan 2012; Ďureje et al. 2012; Macholan et al. 2019). Musculus strains: SKA, Kaerepere, Estonia; SKE, Keava, Estonia; BULS and BUSNA, Buškovice, Czech Republic; PWD, Kunratice (Prague), Czech Republic; STUF and STUS, Studenec, Czech Republic; SENK, Šenkvice, Slovak Republic. Domesticus strains: STRA and STRB, Straas, Bavaria, Germany; STAIL and SCHUNT, Schweben, Hessen, Germany; SPLY, Plössen, Bavaria, Germany; SIN and SIT, Scar, Sanday Island, Orkneys, Scotland. The blank map of Europe by courtesy of Wikimedia Commons (https://en.wikipedia.org/wiki/File:Europe_blank_laea_location_map.svg#filehistory). (B) PRDM9 zinc finger arrays of all strains. The first, nonvariant ZnF separated from ZnF domain is not shown. Three DNA-binding amino acids represent each ZnF. Included are allelic variants of reference strains B6 and PWD. (C) Phylogenetic relationships between Prdm9 alleles estimated using the neighbor-joining method in MEGA X (Kumar et al. 2018). The numbers next to each node represent bootstrapping values.