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The 24 h biofilm of (A) K. pneumoniae, (B) S. epidermidis and (C) P. aeruginosa were incubated with D-LL-31 alone and in combination with antibiotics (AMX or TOB) for 24 h. Biofilm were stained with Alexa Fluor® 488. Biomass was analyzed using COMSTAT and values are shown as mean ± SD in duplicate from three independent experiments. *P<0.05, **P<0.01 and ***P<0.001. Scale bar indicate 100 μm.
