Gene | Primers (F/R) | TA (°C) | Product (bp) | CpG sites | Genomic Location |
MTHFR | F 5’–GGTCAGAAGCATATCAGTCATGAG– 3’ | 58° | 213 bp | 6 | Chr1: 11,785,723–11,805,413) |
R 5’–CTGGGAAGAACTCAGCGAACTCA– 3’ | |||||
LPL | F 5’–GATGTTGAATATTGAATTATTTTAGTAATA– 3’ | 63° | 235 bp | 4 | Chr8: 19,939,253–19,967,259) |
R 5’–AAAAATCACCATACCCAACTAATTTT– 3’ | |||||
ADRB3 | F 5’–TAGGTGATTTGGGAGATTTTTTTT– 3’ | 60° | 238 bp | 4 | Chr8: 37,962,990–37,966,599 |
R 5’- CCCCTAACA ACCCACTAATATTAAC– 3’ |
F–forward; R–reverse; Ta−annealing temperature.