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. 2020 Dec 16;15(12):e0239989. doi: 10.1371/journal.pone.0239989
Gene Primers (F/R) TA (°C) Product (bp) CpG sites Genomic Location
MTHFR F 5’–GGTCAGAAGCATATCAGTCATGAG– 3’ 58° 213 bp 6 Chr1: 11,785,723–11,805,413)
R 5’–CTGGGAAGAACTCAGCGAACTCA– 3’
LPL F 5’–GATGTTGAATATTGAATTATTTTAGTAATA– 3’ 63° 235 bp 4 Chr8: 19,939,253–19,967,259)
R 5’–AAAAATCACCATACCCAACTAATTTT– 3’
ADRB3 F 5’–TAGGTGATTTGGGAGATTTTTTTT– 3’ 60° 238 bp 4 Chr8: 37,962,990–37,966,599
R 5’- CCCCTAACA ACCCACTAATATTAAC– 3’

F–forward; R–reverse; Ta−annealing temperature.