. 2020 Dec 16;15(12):e0243499. doi: 10.1371/journal.pone.0243499

Table 1. Expression of genes related to proliferation, differentiation, and apoptosis.

	Young		Old		Significance
	Control	Ethanol	Control	Ethanol	Age	Treatment	Interaction
Ccnd1	2.38 ± 0.41	1.56 ± 0.39	2.33 ± 0.86	1.33 ± 0.41	0.794	0.095	0.878
Cdk2	1.62 ± 0.32^a	0.69 ± 0.14^b	1.65 ± 0.53^a	1.02 ± 0.22^b	0.569	0.017	0.633
Cdk4	2.37 ± 0.38^a	1.80 ± 0.53^a	0.51 ± 0.10^b	0.67 ± 0.17^b	0.001	0.621	0.376
Cdkn1a	1.23 ± 0.21	1.27 ± 0.36	0.99 ± 0.22	2.35 ± 0.71	0.305	0.095	0.116
Cdh1	1.16 ± 0.15^a	0.83 ± 0.11^a	0.52 ± 0.18^b	0.59 ± 0.23^b	0.013	0.450	0.237
p53	2.37 ± 0.47^a	1.75 ± 0.46^a	0.69 ± 0.16^b	1.01 ± 0.40^b	0.005	0.714	0.260

Values expressed as means ± standard errors. Numbers with different superscripts differ significantly at P < 0.05. Data were analyzed using two-way ANOVA, with follow up post hoc t-tests. Young/Control (N = 12); Young/Ethanol (N = 12); Old/Control (N = 10); Old/Ethanol (N = 10).

Ccnd1: Cyclin D1; Cdk2: Cyclin dependent kinase 2; Cdk4: Cyclin dependent kinase 4; Cdkn1a: p21^waf1/cip1, Cdh1: E-cadherin.