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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Anal Chem. 2020 Aug 11;92(17):11935–11942. doi: 10.1021/acs.analchem.0c02338

Figure 2.

Figure 2.

(A) SDS-PAGE of Nb-3G2, Nb-4D11 and Nb-3F9. (B) Standard curves of ic-ELISA based on Nb-3G2, Nb-4D11, and Nb-3F9. (C) SDS-PAGE of Nb-3F9-Nluc, (D) Evaluation of nanoluciferase catalytic activity, nanobody binding activity, and specific TeA inhibition activity of Nb-3F9-Nluc. The nanoluciferase catalytic activity of Nb-3F9-Nluc was determined in the presence or absence of bioluminescent substrate CTZ-h, the nanobody binding activity of Nb-3F9-Nluc was determined in the presence or absence of coating antigen by direct noncompetitive BLEIA, and the specific TeA inhibition activity of Nb-3F9-Nluc was determined in the presence or absence of TeA by direct competitive BLEIA.