FIGURE 5.

MAFG‐AS1 could promote PTBP1 translation by increasing its expression through enhancing Hu antigen R (HuR) stability. A, The expression of PTBP1 protein was detected by Western blot in bladder urothelial carcinoma (BUC) cell lines (BIU87, 5637, T24, EJ, and RT4). B, Western blot assay detected the expression of HuR in tumor and adjacent tissues. C, Immunohistochemical detection of PTBP1 protein expression in tumor tissues and adjacent tissues of BUC patients. D, The catRAPID database predicted that HuR is much more likely to bind to PTBP1 mRNA (discriminative power [DP] ranges from 0% (unpredictability) to 100% (predictability); DP values above 50% indicate that the interaction is likely to take place, whereas DPs above 75% represent high‐confidence predictions). E, HuR is positively correlated with PTBP1 in BUC based on the GEO dataset (GSE124305, GSE87304). F, RIP assay was conducted to detect whether HuR protein can bind to PTBP1 mRNA. G, The effect of MAFG‐AS1 and HuR on PTBP1 mRNA levels was detected by RT‐qPCR. H and I, Western blot detected the expression of PTBP1 protein. J, The stability of PTBP1 mRNA was measured by RNA stability assay in each experimental group. Bars represent standard deviation, ns P > .05, *P < .05, **P < .01, ***P < .001