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. 2020 Dec 16;10(8):e241. doi: 10.1002/ctm2.241

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© 2020 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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MAFG‐AS1/HuR/PTBP1 axis can promote proliferation, invasion, metastasis, and epithelial‐mesenchymal transition (EMT) of bladder urothelial carcinoma (BUC) in vivo. A and B, Colony formation assay (A) and MTT assay (B) were used to examine the effect of MAFG‐AS1/HuR/PTBP1 axis on cell proliferation. C and D, The effect of MAFG‐AS1/HuR/PTBP1 axis on cell metastasis and invasion was examined by wound‐healing assay (C) and transwell assay (D). E, The expression of HuR, PTBP1, E‐cadherin, and vimentin was examined by Western blot. Bars represent standard deviation, ns P > .05, *P < .05, **P < .01, ***P < .001