Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) |
BL21 Star (DE3) pRARE | EMBL Heidelberg Core Facility |
Electrocompetent cells |
|
| Cell line (Spodoptera frugiperda) |
IPLB-Sf21-AE | Gibco | ||
| Cell line (Trichoplusia ni) | BTI-Tn-5B1-4 | Gibco | ||
| Strain, strain background (Saccharomyces cerevisiae) |
BY4741 (MATa) yeast | Euroscarf | Y00000 | |
| Strain, strain background (Saccharomyces cerevisiae) |
BY4743 (MATa/α) yeast | Euroscarf | Y20000 | |
| Antibody | Anti-protein-A IgG (mouse, monoclonal) |
Sigma Aldrich | P2921 | (1:333 dilution) |
| Recombinant DNA reagent | Tho2 | This paper (Materials and methods) |
Uniprot P53552 |
pFastBac Hta-Tho2 Conti Lab |
| Recombinant DNA reagent | Hpr1 | This paper (Materials and methods) |
Uniprot P17629 |
pFastBac Hta-Hpr1 Conti Lab |
| Recombinant DNA reagent | Mft1 | This paper (Materials and methods) |
Uniprot P33441 |
pFastBac Hta-Mft1 Conti Lab |
| Recombinant DNA reagent | Thp2 | This paper (Materials and methods) |
Uniprot O13539 |
pFastBac Hta-Thp2 Conti Lab |
| Recombinant DNA reagent | Tex1 | This paper (Materials and methods) |
Uniprot P53851 |
pFastBac Hta-Tex1 Conti Lab |
| Recombinant DNA reagent | Sub2 | This paper (Materials and methods) |
Uniprot Q07478 |
3C-GST-fusion Conti Lab |
| Recombinant DNA reagent | GFP-Sub2 | This paper (Materials and methods) |
Conti Lab | |
| Commercial assay or kit | Bac-to-Bac Baculovirus Expression System |
ThermoFisher Scientific | ||
| Software, algorithm | SerialEM | https://bio3d.colorado.edu/SerialEM/ | SerialEM_3-8- 0beta8_64 & SerialEM_3-8- 0beta11_64 |
|
| Software, algorithm | Focus |
https://focus.c-cina.unibas.ch/
wiki/doku.php |
v 1.1.0 | |
| Software, algorithm | cryosparc | doi: 10.1038/nmeth.4169 | Cryosparc2 | |
| Software, algorithm | CTFfind4 | doi: 10.1016/j.jsb.2015.08.008 | ||
| Software, algorithm | TOPAZ | doi: 10.1038/s41592-019-0575-8 | ||
| Software, algorithm | UCSF Chimera | UCSF, https://www.cgl.ucsf.edu/chimera/ |
||
| Software, algorithm | UCSF ChimeraX | UCSF, https://www.rbvi.ucsf.edu/chimerax/ |
||
| Software, algorithm | COOT |
http://www2.mrc-lmb.cam.ac.uk/
personal/pemsley/coot/ |
0.9 | |
| Software, algorithm | Phenix | https://www.phenix-online.org/ | PHENIX 1.18 | |
| Software, algorithm | Molprobity | Duke Biochemistry, http://molprobity.biochem.duke.edu/ |
||
| Software, algorithm | PyMol 2 | PyMOL Molecular Graphics System, Schrodinger LLC |
PyMOL 2.1 |